产品简介：
Zeocin是常用的高效筛选抗生素，适合多种细胞类型筛选表达Sh ble基因的载体（细菌、真核微生物、植物细胞和动物细胞）。
Zeocin是从轮枝链霉菌（Streptomyces CL990）分离得到的一种铜螯合的糖肽抗生素，通过嵌入DNA使其断裂而导致细胞死亡。Zeocin可在大多数需氧细胞中发挥功效。
分子式：C55H83N19O21S2Cu 分子量：1137.41
溶解性：溶于水，溶于HEPES缓冲液。
溶液配制方法：将zeocin 粉末溶于HEPES buffer (5 g/l, pH 7.2+/- 0.1)，浓度为100mg/mL。然后用0.22微米滤膜过滤。
- 生物活性：
- Zeocin可选择Sh ble基因表达的细胞。与现在使用的其他动物细胞标记物无交叉抗性。因此这种抗生素可用来分离对其他筛选剂（如：庆大霉素，潮霉素）有抗性的克隆。Zeocin是一种属于争光霉素家族的糖蛋白抗生素，在体内能作用于大多数细菌（包括E. coli）、真菌（如：酵母菌）、植物细胞、动物细胞。 液体Zeocin可用于细胞培养，用于筛选转化株的Zeocin浓度根据pH值和盐浓度改变。pH越高，盐浓度越低，Zeocin活性越大。 普通大肠杆菌菌株筛选可在25 μg/ml zeocin浓度的低盐LB琼脂培养基中进行，pH调至7.5。
WORKING CONCENTRATIONS
Zeocin™ is normally used at a concentration of 100 μg/ml, a 1000-fold dilution from the stock solution. However, the optimal concentration
needs to be determined for your cells. Suggested concentrations of Zeocin™ for selection in some examples of mammalian cells are listed below:
| Cell line | Medium | Zeocin® conc | References |
|---|---|---|---|
| B16 (Mouse melanocytes) | RPMI | 20-250 μg/ml | 1-3 |
| CHO (Chinese hamster ovarian cells) | DMEM | 100-500 μg/ml | 1, 4, 5 |
| COS (Monkey kidney cells) | DMEM | 100-400 μg/ml | 6, 7 |
| HEK293 (Human embryonic kidney cells) | DMEM | 100-400 μg/ml | 8, 9 |
| HeLa (Human uterine cells) | DMEM | 50-100 μg/ml | 10, 11 |
| J558L (Mouse melanocytes) | RPMI | 400 μg/ml | 12 |
| MCF-7 (Human breast adenocarcinoma cells) | DMEM | 100-400 μg/ml | 13, 14 |
| MEFs (Mouse embryonic fibroblasts) | DMEM | 200-400 μg/ml | 15, 16 |
| THP-1 (Human monocytes) | RPMI | 200 μg/ml | 17 |
References:
1. Bouayadi K. et al., 1997. Overexpression of DNA polymerase beta sensitizes mammalian cells to 2’,3’ deoxycytidine and 3’-azido-3’-deoxythymidine. Cancer Res. 57: 110-116.
2 Hirose Y. et al., 2012. Inhibition of Stabilin-2 elevates circulating hyaluronic acid levels and prevents tumor metastasis. PNAS, 109: 4263 – 4268.
3. Fan H. et al., 2012. Intracerebral CpG immunotherapy with carbon nanotubes abrogates growth of subcutaneous melanomas in mice. Clin Cancer Res.18(20):5628-38.
4. Li F. et al., 1996. Post-translational modifications of recombinant P-selection glycoprotein ligand-1 required for binding to P- and E- selection. J. Biol. Chem. 271: 3255-3264.
5. Ogura T. et al., 2004. Resistance of B16 melanoma cells to CD47-induced negative regulation of motility as a result of aberrant N-glycosylation of SHPS-1. J Biol Chem. 279(14):13711-20.
6. Saxena A. et al., 2002. H2, the minor subunit of the human asialoglycoprotein receptor, trafficks intracellularly and forms homo-oligomers, but does not bind asialo-orosomucoid. J Biol Chem. 277(38):35297-304.
7. Kanamori A. et al., 2002. Distinct sulfation requirements of selectins disclosed using cells that support rolling mediated by all three selectins under shear flow. L-selectin prefers carbohydrate 6-sulfation totyrosine sulfation, whereas p-selectin does not. J Biol Chem. 277(36):32578-86.
8. Ahmed et al., 2013. TRIF-mediated TLR3 and TLR4 signaling is negatively regulated by ADAM15. J Immunol. 190(5):2217-28.
9. Büllesbach EE. & Schwabe C., 2006. The mode of interaction of the relaxin-like factor (RLF) with the leucine-rich repeat G protein-activated receptor 8. J Biol Chem. 281(36):26136-43.
10. Mesnil M. et al., 1996. Bystander killing of cancer cells by herpes simplex virus thymidine kinase gene is mediated by connexins. PNAS 93(5):1831-5.
11. Maszczak-Seneczko D. et al., 2013. UDP-N-acetylglucosamine transporter (SLC35A3) regulates biosynthesis of highly branched N-glycans and keratan sulfate. J Biol Chem. 288(30):21850-60.
12. Cedeno-Laurent F. et al., 2010. Development of a nascent galectin-1 chimeric molecule for studying the role of leukocyte galectin-1 ligands and immune disease modulation. J Immunol. 185(8):4659-72.
13. Kim HS. et al., 2004. Insulin-like growth factor-binding protein 3 induces caspase-dependent apoptosis through a death receptor-mediated pathway in MCF-7 human breast cancer cells. Cancer Res. 64(6):2229-37.
14. List HJ. et al., 2001. Ribozyme targeting demonstrates that the nuclear receptor coactivator AIB1 is a rate-limiting factor for estrogen-dependent growth of human MCF-7 breast cancer cells. J Biol Chem. 276(26):23763-8.
15. Waak J. et al., 2009. Oxidizable residues mediating protein stability and cytoprotective interaction of DJ-1 with apoptosis signal-regulating kinase 1. J Biol Chem. 284(21):14245-57.
16. Maue A. et al., 2013. The polysaccharide capsule of Campylobacter jejuni modulates the host immune response. Infect Immun. 81(3):665-72
储存条件：
粉末2-8°C，
运输条件：
常温运输
- 【注意】
- ●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。