别名：EGF; Epidermal Growth Factor; Urogastrone
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Human EGF (Asn971-Arg1023)
Accession # P01133 |
| Predicted molecular mass | 6.2 kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.01 EU/μg by the LAL method |
| Activity | Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤0.3 ng/mL. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Human EGF (Cat. No. MGC015) SDS-PAGE under reducing (R) & Non-reducing conditions.
The gel was stained with MGC015 SDS-PAGE.
Bioactivity
Recombinant Human EGF (Cat. No. MGC015) stimulates proliferation of NIH3T3 cells.
The ED50 for this effect ≤0.3 ng/mL.
The acivity of MGC015 was equivalent to other competing products.
Bioactivity
Measured in a cell proliferation assay using NIH3T3 cells
SEC-HPLC
