别名:Noggin; NOG
基本信息:
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Mouse | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Mouse Noggin (Q28-C232)
Accession # P97466 |
| Predicted molecular mass | 23.1kDa |
| Appearance | White powder,Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining) |
| Endotoxin | ≤0.01EU/μg by the LAL method |
| Activity | Measured by its ability to inhibit 5.0ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 is ≤0.1 μg/ml. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS,5% mannitol and 0.01% Tween 80, pH7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示:
Bioactivity
Measured by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells.

