别名：Vitronectin; VN; S-Protein; Serum-Spreading Factor; V75; VTN
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.001 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Human Vitronectin (Asp20-Leu478)
Accession #P04004 |
| Predicted molecular mass | 52.3kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.001EU/μg by the LAL method |
| Activity | Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 μg/mL.
Optimal concentration depends on cell type as well as the application or research objectives. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Human Vitronectin (Cat. No. MGC089) SDS-PAGE under reducing (R) & Non-reducing conditions.
The gel was stained with MGC089 SDS-PAGE.
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 2-10 μg/mL.
The activity of MGC089 was higher than other competing products.