别名：MG/VAIL C119418; MG/VAIL C119419; MG/VAIL C119420; protein Wnt-3a; wingless-type MMTV integration site family, member 3A; Wnt3a; Wnt-3a
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.1 EU/μg | ≥95% | C-Fc |
| Source | CHO |
| Description
|
Human Wnt-3a (Ser19-Lys352) with a C-terminal linker GGGGGS and Human lgG1 (Asp104-Lys330)
Accession # NP_149122 |
| Predicted molecular mass | 58.2kDa |
| Appearance | White powder,Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining) |
| Endotoxin | ≤0.1EU/μg by the LAL method |
| Activity | Measured by its ability to induce Topflash reporter activity in CHO-K1 cells. The ED50 for this effect is ＜30ng/ml. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, pH7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
Bioactivity
Measured by its ability to induce Topflash reporter activity in CHO cells in the presence of 5ng/ml BMP2.
The ED50 for this effect is < 30ng/ml.
The activity of Recombinant Human Wnt-3a (hFc Tag )(Cat. No.MGC090) was higher than other competing products.
