别名：Tumor Necrosis Factor; TNF-Alpha
基本信息：
| Source | HEK293 |
| Species | Human |
| Tag | C-AviTag-His |
| Description | Biotinylated Human TNFα (Val77 – Leu233) |
| Accession# | P01375 |
| Predicted molecular mass | ~20.3 kDa. It is composed of one non-glycosylated polypeptide chain containing 157 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.005 EU/μg |
| Activity | Immobilized Recombinant Biotinylated Human TNFα (His,AviTag) at 1μg/ml
(100μl/well) can bind Infliximab (Cat. No. MB2773) with a linear range of 1-20ng/ml. |
| Formulation | 0.2 μm-filtered solution containing PBS with 0.01% Tween 80, trehalose and mannitol, pH 7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240（1mL无菌水+10mL稀释液） |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Biotinylated Human TNFα (Cat. No. MGC094) on SDS-PAGE under non-reducing (NR) and reducing (R) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of MGC094 is more than 95% verified by SEC-HPLC.
Bioactivity
Immobilized Recombinant Biotinylated Human TNFα at 1μg/ml (100μl/well) can bind Infliximab (Cat. No. MB2773) with a linear range of 1-20ng/ml. The EC50 is 0.01029μg/ml.