别名：Tumor Necrosis Factor; TNF-Alpha
基本信息：
| Source | HEK293 |
| Species | Mouse |
| Tag | C-His |
| Description | Mouse TNFα (Leu 80 – Leu 235) |
| Accession# | P06804 |
| Predicted molecular mass | ~18.4 kDa. It is composed of one non-glycosylated polypeptide chain containing 156 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.005 EU/μg |
| Activity | Immobilized Mouse TNFα (Cat. No. MGC095) at 3μg/ml (100μl/well) can bind Mouse TNFR1-hFc with a linear range of 3-30ng/ml. |
| Formulation | 0.2 μm-filtered solution containing PBS with 0.01% Tween 80, trehalose and mannitol, pH 7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240（1mL无菌水+10mL稀释液） |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Mouse TNFα (Cat. No. MGC095) on SDS-PAGE under non-reducing (NR) and reducing (R) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-HPLC
The purity of MGC095 is more than 95% verified by SEC-HPLC.
Bioactivity
Immobilized Mouse TNFα (Cat. No. MGC095) at 3μg/ml (100μl/well) can bind Mouse TNFR1-hFc with a linear range of 3-30ng/ml. The EC50 is 0.02205μg/ml.