别名:DER6; GIF; L-dopachrome Isomerase; Macrophage migration inhibitory factor
基本信息:
| Source | E. coli. |
| Species | Human |
| Tag | Tag free |
| Description | Human MIF (Met1- Ala115) |
| Accession# | P14174 |
| Predicted molecular mass | ~12.5 kDa. It is composed of one non-glycosylated polypeptide chain containing 115 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.1 EU/μg |
| Activity | The specific activity is determined by binding rhCD74 in a functional ELISA. |
| Formulation | 0.2 μm-filtered solution containing 20 mM PB, with 150 mM NaCl, 0.02% (v/v) Tween-80 and 5% (v/v) Trehalose, pH 7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240(1mL无菌水+10mL稀释液) |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示:
SDS-PAGE

Recombinant Human MIF (Cat. No. MGC168)SDS-PAGE under reducing (R) & Non-reducing conditions.

