别名:NNT-1; B-Cell Stimulating Factor-3; BSF-3
基本信息:
| Source | E. coli. |
| Species | Human |
| Tag | Tag free |
| Description | Human Novel Neurtrophin-1/BCSF-3 (Leu28-Phe225) |
| Accession# | Q9UBD9 |
| Predicted molecular mass | ~22.3 kDa. It is composed of one non-glycosylated polypeptide chain containing 198 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.1 EU/μg |
| Activity | The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of ≥ 2.5×10^5 IU/mg in the presence of human CNTF R alpha |
| Formulation | 0.2 μm-filtered solution containing 30% Acetonitrile and 0.1% TFA. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240(1mL无菌水+10mL稀释液) |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示:
SDS-PAGE
Recombinant Human Novel Neurtrophin-1/BCSF-3 (Cat. No. MGC170)SDS-PAGE under reducing (R) & Non-reducing conditions.
Bioactivity

The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of ≥ 2.5×10^5 IU/mg in the presence of human CNTF R alpha

