别名：RSPO1; R-spondin1; R-spondin-1
基本信息：
| Source | E. coli. |
| Species | Mouse |
| Tag | Tag free |
| Description | Mouse R-spondin 1 (Ser21-Gly209) |
| Accession# | Q9Z132 |
| Predicted molecular mass | ~20.9 kDa. It is composed of one non-glycosylated polypeptide chain containing 189 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.01 EU/μg |
| Activity | Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. |
| Formulation | 0.2 μm-filtered solution containing 20 mM PB, with 150 mM NaCl, pH 7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240（1mL无菌水+10mL稀释液） |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Mouse R-spondin 1 (Cat. No. MGC188)SDS-PAGE under reducing (R) & Non-reducing conditions.