别名：Interleukin-6; BSF-2; CDF; Hybridoma growth factor; IFN-beta-2
基本信息：
| Source | E. coli. |
| Species | Rat |
| Tag | Tag free |
| Description | Rat IL-6 (Phe25-Thr211) |
| Accession# | P20607 |
| Predicted molecular mass | ~21.7 kDa. It is composed of one non-glycosylated polypeptide chain containing 187 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.1 EU/μg |
| Activity | The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of ≥ 1.0×10^8 IU/mg. |
| Formulation | 0.2 μm-filtered solution containing 20 mM PB, with 150 mM NaCl, 5% (v/v) Trehalose, pH 7.4. |
| Reconstitution | It is recommended to redissolve in 10 mM HCl. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL241（1mL 4mM HCl+10mL稀释液） |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Rat IL-6 (Cat. No. MGC195)SDS-PAGE under reducing (R) & Non-reducing conditions.
Bioactivity
The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/ml, corresponding to a specific activity of ≥ 1.0×10^8 IU/mg.