Ham’s F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
|• L-Glutamine 谷氨酰胺(1mM)||• HEPES|
|• Phenol Red 酚红(1.3mg/L)||• Penicillin & Streptomycin 双抗|
|• Sodium Pyruvate 丙酮酸钠(110mg/L)|