产品简介：
Actinomycin D是一种多肽抗生素，与双链DNA结合形成稳定复合物，从而抑制DNA修复，还会导致DNA的单链断裂，抑制RNA的合成，作用于mRNA干扰细胞的转录过程，还可使细胞周期停滞在G1期，进而抑制细胞增殖。
分子式：C62H86N12O16 分子量：1255.42
物理性状及指标：
产品形式：………………… 鲜红色结晶粉末
溶解性：…………………… DMSO: 20 mg/mL
Assay：…………………………≥98%,BR
用途及描述：
- 抑制肿瘤形成
- 可以用于手术后化疗，与长春新碱, 环磷酰胺等合用治疗间充质细胞瘤
| 来源公开文献仅供参考 | |
| IC50 & Target | DNA repair |
|---|---|
| In Vitro | Neuroblastoma cell lines with different p53 genetic background were employed to determine the response on cell viability and apoptosis of low-dose of actinomycin D. Subcutaneously-implanted SK-N-JD derived neuroblastoma tumors were used to assess the effect of low-doses of actinomycin D on tumor formation[1]. |
| In Vivo |
Three animals received an intravenous bolus injection of 0.5mg/kg actinomycin D in 0.125 mL per mouse (25 g) of a 0.1 mg/mL dosing solution.This dosage corresponds to the usual therapy protocols in humans according to allometric scaling . Full blood samples were transferred into citrate tubes and centrifuged at 1600× g for 10 min to generate plasma samples, which were stored at −80 °C until analysis. For determination of actinomycin D disposition in the CNS, brain tissue, and plasma samples were harvested following euthanasia with increasing concentrations of CO2 at 2 h (three animals) or 22 h (four animals) after intravenous bolus injection of 0.5 mg/KG actinomycin D. Samples were stored at −80 °C until analysis. For validation of the UPLC-MS/MS quantification, blank plasma from 6 naïve mice was used and blank brain tissue was obtained from 2 unexposed mice. Whole brain tissue was homogenized using a Bead Ruptor 4 homogenizer in acetonitrile (ACN)/water (1/19, v/V) + 0.5 % Triton X and 0.1 % formic acid (FA) (100 mg brain tissue/mL) in 2.0 mL tubes containing ~25 glass beads for 2 × 1 min[2]. |
| 参考文献 |
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储存条件：
-20℃，避光防潮密闭干燥
关键词: 放线菌素 D
- 【注意】
- ●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。