Blasticidin(灭瘟素S盐酸盐)

¥600.00

规格: 10mg

CAS 号:3513-03-9
英文名字:Blasticidine S hydrochloride
质量标准:HPLC>95%,BR

库存详情未设置 SKU: MB2506-1 分类: , ,

Blasticidin灭瘟素S盐酸盐
分子式:C17H26N8O5·HCl,分子量:458.90
物理性质及指标:
外观:……………………粉末
纯度:……………………>95% (HPLC)
溶解性:…………………溶于水>10mg/ml

使用方法推荐(仅供参考)
储运液配置:溶解于无菌细胞培养级1M HEPES buffer (MA0036)或者PBS1X(MA0015),配置成浓度为10mg/ml. 无菌微膜过滤。然后根据自身实验要求进行稀释或者使用
筛选条件
– Escherichia coli(大肠杆菌)
E. coli is poorly sensitive to blasticidin, but transformants resistant to blasticidin can be selected on low salt LB agar medium (pH 8) supplemented with 100 μg/ml blasticidin. High pH enhances the activity of blasticidin.

– Mammalian cells(哺乳动物细胞)
The working concentration of blasticidin for mammalian cell lines varies from 1 to 10 μg/ml, in a few cases up to 30 μg/ml. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. After treatment, cell death occurs rapidly, allowing the selection of transfected cells with plasmids carrying the bsr or BSD genes in as little as 7 days post-transfection. Suggested concentrations of blasticidin for selection in some examples of mammalian cells are listed below.

Cell line

Medium

Blasticidin conc

CHO (Chinese hamster ovarian cells)

DMEM

5-10 μg/ml

HEK293 (Human embryonic kidney cells)

DMEM

5-15 μg/ml

HeLa (Human uterine cells)

DMEM

2.5-10 μg/ml

Neuro2a (Mouse neuroblasts)

DMEM

30 μg/ml

THP-1 (Human monocytes)

RPMI

10 μg/ml

用途及描述:科研试剂,灭瘟素S属于抗生素,具有真菌属性,通过研究肽键的形成,可以抑制细菌和真核生物体内蛋白质合成,并且可以有效防止稻瘟病病。灭瘟素S常被用作含有抗癌基因bls, bsr, or BSD癌变细胞的选择性试剂。
储存条件:-20℃,避光防潮密闭干燥。

运输条件:2~8℃运输
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规格

10mg

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