分子式：C55H85O21N20S2Cu·HCl 分子量：1526.5
简介：Zeocin是腐草霉素D1(Phleomycin D1)的商业名称，是从轮枝链霉菌(Streptomyces CL990)分离得到的一种铜螯合的糖肽抗生素，属于博来霉素(bleomycin)家族，通过嵌入DNA使其断裂而导致细胞死亡。Zeocin可在大多数需氧细胞中发挥功效。Zeocin是常用的高效筛选抗生素，适合多种细胞类型筛选表达Sh ble基因的载体（细菌、真核微生物、植物细胞和动物细胞）。
别名：Zeocin powder；Phleomycin D1；腐草霉素D1
物理性质和指标：
外观：……………………蓝色固体
溶解性：…………………溶于水、HEPES缓冲液
用途及描述：科研试剂，广泛应用于分子生物学，药理学等科研方面，严禁用于人体。主要用于以下用途：
- 用于基因敲除和基因沉默研究。
- 用于细胞培养中的抗生素筛选。
- 用于研究DNA损伤和修复机制。
- 用于研究细菌耐药性。
储存条件：2-8℃，避光防潮密闭干燥
运输条件：湿冰运输（按季节）
生物活性：
Zeocin可选择Sh ble基因表达的细胞。与现在使用的其他动物细胞标记物无交叉抗性。因此这种抗生素可用来分离对其他筛选剂（如：庆大霉素，潮霉素）有抗性的克隆。Zeocin是一种属于争光霉素家族的糖蛋白抗生素，在体内能作用于大多数细菌（包括E.coli）、真菌（如：酵母菌）、植物细胞、动物细胞。液体Zeocin可用于细胞培养，用于筛选转化株的Zeocin浓度根据pH值和盐浓度改变。pH越高，盐浓度越低，Zeocin活性越大。普通大肠杆菌菌株筛选可在25μg/mL zeocin浓度的低盐LB琼脂培养基中进行，pH调至7.5。
经典实验操作（来源于公开文献，仅供参考）
- 储液配制：将Zeocin粉末溶于HEPES buffer(5 g/L,pH:7.2±0.1)，浓度为100mg/mL。然后用0.22微米滤膜过滤。
- 筛选条件：Zeocin通常以100μg/mL的浓度使用，即从储备液稀释1000倍所得。然而对不同细胞，最佳浓度需要确定。下表列出了在某些哺乳动物细胞中进行筛选时Zeocin的建议浓度。
| Cell line | Medium | Blasticidin conc |
| B16 (Mouse melanocytes) | RPMI | 20-250μg/mL |
| CHO (Chinese hamster ovarian cells) | DMEM | 100-500μg/mL |
| COS (Monkey kidney cells) | DMEM | 100-400μg/mL |
| HEK293 (Human embryonic kidney cells) | DMEM | 100-400μg/mL |
| HeLa (Human uterine cells) | DMEM | 50-100μg/mL |
| J558L (Mouse melanocytes) | RPMI | 400μg/mL |
| MCF-7 (Human breast adenocarcinoma cells) | DMEM | 100-400μg/mL |
| MEFs (Mouse embryonic fibroblasts) | DMEM | 200-400μg/mL |
| THP-1 (Human monocytes) | RPMI | 200μg/mL |
【注意】
- 溶解性是在室温下测定的，如果温度过低，可能会影响其溶解性。
- 我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
- 为了您的安全和健康，请穿实验服并戴一次性手套操作。
- 部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。
参考文献：
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关键词
Phleomycin, 博来霉素