简介：XTTis used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble XTT to a water-soluble, orange colored formazan product. Unlike MTT, XTT does not require solubilization prior to quantitation, thereby reducing the assay time in many viability assay protocols. Moreover, the sensitivity of the XTT reduction assay is reported to be similar to or better than that of the MTT reduction assay.
XTT is a colorimetric assay used to assess cell viability as a function of cell number based on metabolic activity. This rapid, sensitive, non-radioactive assay is detected using standard microplate absorbance readers.
1.Grow cells in a 96-well plate at a density of 104–105 cells/well in 100 µL of culture medium with compounds to be tested. Culture in a CO2 incubator for 24–48 hours
2.Make a 10 mM PMS solution in phosphate-buffered saline (3 mg PMS into 1 mL PBS)；PMS美仑货号MB6047
3.Dissolve 4 mg of XTT in 4 mL of 37°C cell culture medium
4.Add 10 µL of the PMS solution the 4 mL of XTT solution created in step 3immediatelybefore labeling cells
5.Add 25 µL of XTT/PMS solution directly to each well containing 100 µL cell culture
6.Incubate for 2 hours at 37°C in a CO2 incubator
7.Read absorbance at 450 nm