别名：Dickkopf-related protein 1; Dickkopf-1; Dkk-1
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Human Dkk-1 (Thr32-His266)
Accession # O94907 |
| Predicted molecular mass | 25.7kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.01 EU/μg by the LAL method |
| Activity | Measured by its ability to inhibit 50ng/ml wnta-3a induced Topflash reporter activity in CHO cells. The ED50 is ≤50 ng/ml. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
Bioactivity
Measured by its ability to inhibit 50ng/ml Wnt-3a induced Top flash reporter activity in CHO cells.