别名：Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF; Molgramostin; Sargramostim; CSF2; GMCSF
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Human GM-CSF(Ala18-Glu144)
Accession # P04141 |
| Predicted molecular mass | 14.5 kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.01EU/μg by the LAL method |
| Activity | Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is ≤0.2 ng/mL. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Human GM-CSF (Cat. No. MGC025) SDS-PAGE under reducing (R) & Non-reducingconditions.
The gel was stained with MGC025 SDS-PAGE.
Bioactivity
Recombinant Human GM-CSF (Cat. No. MGC025) stimulates proliferation of TF-1 cells.
The ED50 for this effect is ≤0.2 ng/mL.
Compared with other competitors in terms of bioactivity, MGC025 is equivalent to competitor # 1 >competitor # 2>competitor # 3.
SEC-HPLC
