别名：Interleukin-4; IL-4; BSF-1; Binetrakin; Pitrakinra; IL4
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Human Interleukin-4 (His25-Ser153)
Accession # P05112 |
| Predicted molecular mass | 15 kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) condition, visualized by Coomassie staining) |
| Endotoxin | ≤0.01EU/μg by the LAL method |
| Activity | Measured in a cell proliferation assay using TF-1 cells, The ED50 for this effect is ≤ 0.2 ng/mL，corresponding to a specific activity of ＞1 x 107 units/mg. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Human IL-4 (Cat. No. MGC040) SDS-PAGE under reducing (R) condition.
The gel was stained with MGC040 SDS-PAGE.
Bioactivity
Measured in a cell proliferation assay using TF-1 cells.
Bioactivity
Highly consistent bioactivity between different production batches of Recombinant Human IL-4 (Cat. No. MGC040).
MGC040 stimulates proliferation of TF-1 cells.
The ED50 for this effect is ≤0.2 ng/mL.