别名：Interleukin-12 subunit alpha; IL-12A; CLMF p35; IL-12 subunit p35; NK cell; IL12A
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Recombinant human IL-12 is composed of P40(Ile23-Ser328) and P35(Arg23-Ser219) subunits,which linked by GS-linker（GSGSSRGGSGSGGSGGGGSK）
Accession # P29460(IL12P40), P29459(IL12P35) |
| Predicted molecular mass | 59 kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.01EU/μg by the LAL method |
| Activity | Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
Bioactivity
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells.
The ED50 for this effect is ＜0.5 ng/mL.
The activity of MGC047 was higher than other competing products.