别名：Laminin 521; LN521
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| HEK293 | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | HEK293 |
| Description | Human Laminin521 E8(Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609) Accession #O15230&P55268&P11047 |
| Predicted molecular mass | 141.0 KDa |
| Appearance | White powder,colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R)& Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.01EU/μg by the LAL method |
| Activity | Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 cells. The ED50 for this effect is ≤10 ng/ml. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS,5% Mannitol,0.01% Tween 80,pH7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |