别名：Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; TNF; TNFA; TNFSF2
基本信息：
| 表达系统 | 种属 | 内毒素 | 纯度 | 标签 |
| CHO | Human | ≤0.01 EU/μg | ≥95% | Tag free |
| Source | Chinese Hamster Ovary cell line |
| Description
|
Human TNFα (Val77-Leu233)
Accession # P01375 |
| Predicted molecular mass | 17.4 kDa |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | ≤0.01EU/μg by the LAL method |
| Activity | Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. |
| Formulation | Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4 |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
SDS-PAGE
Recombinant Human TNFα (Cat. No. MGC084) SDS-PAGE under reducing (R) & Non-reducing conditions.
The gel was stained with MGC084 SDS-PAGE.
Bioactivity
Recombinant Human TNFα (Cat. No. MGC084) measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 5-60 pg/mL. The activity of MGC084 was equivalent to other competing products.
Bioactivity
Measured in a cytotoxicity assay using L‑ 929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
SEC-HPLC
