别名：Insulin-like growth factor binding protein 3; IBP-3; IGF-binding protein 3; IGF-BP3
基本信息：
| Source | E. coli. |
| Species | Human |
| Tag | Tag free |
| Description | Human IGFBP-3 (Gly28-Lys291) |
| Accession# | P17936 |
| Predicted molecular mass | ~28.8 kDa. It is composed of one non-glycosylated polypeptide chain containing 264 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.1 EU/μg |
| Activity | The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of ≥ 5.0×10^3 IU/mg in the presence of 15 ng/ml of rHuIGF-II. |
| Formulation | 0.2 μm-filtered solution containing 20 mM PB, with 150 mM NaCl, pH 7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240（1mL无菌水+10mL稀释液） |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
数据展示：
Bioactivity
The ED50 as determined by inhibiting IGF-II induced proliferation of serum free human MCF-7 cells is less than 200 ng/ml, corresponding to a specific activity of ≥ 5.0×10^3 IU/mg in the presence of 15 ng/ml of rHuIGF-II.