别名:Fibroblast Growth Factor 2; FGF-2; HBGF-2
基本信息:
| Source | E. coli. |
| Species | Bovine |
| Tag | Tag free |
| Description | Bovine bFGF (Pro10-Ser155 with an N-terminal Met) |
| Accession# | P03969 |
| Predicted molecular mass | ~16.5 kDa. It is composed of one non-glycosylated polypeptide chain containing 147 amino acids. |
| Appearance | White powder, Colorless clear liquid after reconstitution |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining) |
| Endotoxin | < 0.1 EU/μg |
| Activity | The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0×10^7 IU/mg. |
| Formulation | 0.2 μm-filtered solution containing 20 mM PB, with 150 mM NaCl, pH 7.4. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. 细胞因子溶解、稀释、保存可使用即用型专用溶剂套装—PWL240(1mL无菌水+10mL稀释液) |
| Shipping | Wet ice (seasonal) |
| Storage&Stability
|
36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |

